BacT/ALERT® Culture Media

Strong Outside, Strong Inside

BacT/ALERT® Culture media offers an ideal environment for recovering an array of microorganisms, including bacteria, fungi, and mycobacteria.

  • Media flexibility allows recovery of microorganisms from many sample types, including blood and sterile body fluids
  • Minimizes false negatives using a combination of colorimetric technology and sophisticated algorithms
  • Fastidious Antimicrobial Neutralization media available, in addtion to standard media
  • Plastic bottles are shatter-resistant to maximize safety without compromising performance

Minimize False Negatives

BacT/ALERT® Culture media combines colorimetric technology with sophisticated algorithms to minimize the incidence of false negatives due to delayed entry into the BacT/ALERT® 3D system.  Specialized Liquid Emulsion Sensors (LES) at the bottom of each culture bottle visibly change color in response to shifts in pH as a result of rising CO2 levels produced by microorganisms.  

The BacT/ALERT® 3D instrument measures the color changes every ten minutes and analyzes them using algorithms that include a unique "threshold" algorithm to ensure early detection of microorganisms.  In the event that bottles are delayed in transit, the visible, irreversible color change helps identify potentially positive bottles before they are entered into the system.  In addition to minimizing false negatives, BacT/ALERT® Culture Media also has a low rate of false positives.

Reliable Microorganism Recovery

BacT/ALERT® Culture Media provides a wide range of media bottles to rapidly and reliably recover an array of microorganisms, including bacteria, mycobacteria, fungi, and yeasts from various sample types.  BacT/ALERT® Culture Media is FDA-cleared for blood, sterile body fluids, and platelets, and can detect 98% of isolates within 72 hours.*

  • Fastidious Antimicrobial Neutralization media helps optimize microorganism recovery for patients who are undergoing antimicrobial therapy
  • Specialized bottles designed for recovery of mycobacteria

*Bourbeau PP and Pohlan JK. Three days of incubation may be sufficient for routine blood cultures with BacT/ALERT® FAN® blood culture bottles. J Clin Microbiol. 2001; 39(6): 2079-82.

Improve Safety & Efficiency

BacT/ALERT® Culture Media bottles are made with a unique polycarbonate material and feature a triple-layer, gas-impermeable design that maintains the appropriate aerobic or anaerobic environment for microorganism recovery.  The bottles can help eliminate accidental spills and are shatter-resistant, which helps reduce the potential for biohazard exposure and glass cuts.  BacT/ALERT® bottles are up to 2/3 lighter than glass, can adapt to a variety of protocols, and enable pneumatic transport without a special container.

Optimized Time to Detection and Microorganism Recovery

The next-generation Fastidious Antimicrobial Neutralization Plus media (FAN® Plus) from bioMérieux features optimized time to detection and microorganism recovery with an improved environment that fosters microbial growth for bacteria and yeast.  FAN® Plus media offers effective organism growth from both blood and sterile body fluid samples, along with growth performance guidelines for use in delayed entry conditions.

Fastidious Antimicrobial Neutralization Plus Media (FAN® Plus)

FAN® Plus media with Adsorbent Polymeric Beads (APB) to neutralize antimicrobials

Bottle Type Media Formulation Specimen Type Specimen Volume
BacT/ALERT® FA Plus
Ref: 410851
30 mL of supplemented complex media containing adsorbent polymeric beads Blood or normally sterile body fluid (SBF) Up to 10 mL
BacT/ALERT® FN Plus
Ref: 410852
40 mL of supplemented complex media containing adsorbent polymeric beads Blood or SBF Up to 10 mL
BacT/ALERT® PF Plus
Ref: 410853
30 mL of supplemented complex media containing adsorbent polymeric beads Blood Up to 4 mL

 

Fastidious Antimicrobial Neutralization Media (FAN®)

FAN® media with activated charcoal to neutralize antimicrobials

Bottle Type Media Formulation Specimen Type Specimen Volume
BacT/ALERT® FA FAN® 
Aerobic
Ref: 259791
30 mL peptone-enriched TSB, supplemented with Brain Heart Infusion (BHI) solids and activated charcoal Blood or normally sterile body fluid (SBF) Up to 10 mL
BacT/ALERT® FN FAN®
Anaerobic
Ref: 259793
40 mL peptone-enriched TSB, supllemented with BHI solids and activated charcoal Blood or SBF Up to 10 mL
BacT/ALERT® PF
Pediatric FAN®
Ref: 259794
20 mL peptone-enriched TSB, supplemented with BHI solids and activated charcoal Blood Up to 4 mL

 

Standard Media

Bottle Type Media Formulation Specimen Type Specimen Volume
BacT/ALERT® SA
Standard Aerobic
Ref: 259789
40 mL supplemented tryptic soy broth (TSB) Blood or normally sterile body fluid (SBF) Up to 10 mL
BacT/ALERT® SN
Standard Anaerobic
Ref: 259790
40 mL TSB Blood or SBF Up to 10 mL

 

Mycobacterium Media

Bottle Type Media Formulation Specimen Type Specimen Volume
BacT/AlERT® MP Mycobacteria
Process Ref: 259797
Use with Ref: 259760
10 mL supplemented Middlebrook 7H9 broth Processed speciment or SBF other than blood 0.5 mL

 

Platelet QC Testing

Bottle Type Media Formulation Specimen Type Specimen Volume
BacT/ALERT®
BPA Aerobic Platelet Culture Bottles
Ref: 279018
40 mL supplemented tryptic soy broth (TSB) Leukocyte reduced apheresis platelet (LRAP) units, and both leukocyte reduced single and a pool of up to six (6) units of leukocyte reduced whole blood platelet concentrates (LRWBPC) 4 - 10 mL
BacT/ALERT®
BPN Anaerobic Platelet Culture Bottles
Ref: 279019
40 mL supplemented tryptic soy broth (TSB) Leukocyte reduced apheresis platelet (LRAP) units, and both leukocyte reduced single and a pool of up to six (6) units of leukocyte reduced whole blood platelet concentrate (LRWBPC) 4 - 10 mL

 

Blood Collection

Image Description Product Number Packaging
BacT/ALERT Blood Collection Adapter Cap
Adapter cap for conventional Luer lock style collection sets
Ref: 279012 120 per box

BacT/ALERT Blood Collection Adapter Insert
Adapter insert for collecting blood into evacuated tubes

Ref: 279013 60 per box

VACUETTE® Blood Culture Holder
Neck design accommodates evacuated tubes without the need for an insert. (Female threaded Luer)

Ref: 450181 320 per box

 

bioMérieux provides service and support for BacT/ALERT® Culture Media users, including a hands-on training course and unlimited technical support over the phone.

Below are bioMérieux's recommendations for blood culture collection using the BacT/ALERT® Culture Media bottles.*

Click to Download

Download Blood Culture Booklet

*This information is given as a guideline only and is not intended to be exhaustive.  This document is not legally binding. bioMérieux reserves the right to modify specifications without notice.

 

Standard vs. FAN® Media

Weinstein MP, Mirrett S, Reimer LG, Wilson ML, Smith-Elekes S, Chuard CR, Joho KL, Reller LB. Controlled evaluation of BacT/ALERT standard aerobic and FAN® aerobic blood culture bottles for detection of bacteremia and fungemia. J Clin Microbiol. 1995;33(4):978-981.

Wilson ML, Weinstein MP, Mirrett S, Reimer LG, Feldman RJ, Chuard CR, Reller LB. Controlled evaluation of BacT/ALERT® standard anaerobic and FAN® anaerobic blood culture bottles for the detection of bacteremia and fungemia. J Clin Microbiol. 1995;33(9):2265-2270.

McDonald LC, Fune J, Gaido LB, Weinstein MP, Reimer LG, Flynn TM, Wilson ML, Mirrett S, Reller LB. Clinical importance of increased sensitivity of BacT/ALERT® FAN® aerobic and anaerobic blood culture bottles. J Clin Microbiol. 1996;34(9):2180-2184.

Mirrett S, Everts RJ, Reller LB. Controlled Comparison of Original Vented Aerobic FAN® Medium with New Nonvented BacT/ALERT® FA Medium for Culturing Blood. J Clin Microbiol. 2001;39(6):2098-2101.

Mirrett S, Petti CA, Woods CW, Magadia R, Weinstein MP, Reller LB. Controlled Clinical Comparison of the BacT/ALERT® FN and the Standard Anaerobic SN Blood Culture Medium. J Clin Microbiol. 2004;42(10):4581-4585.

FAN® vs. BACTEC Plus

Ziegler R, Johnscher I, Martus P, Lenhardt D, Just HM. Controlled clinical laboratory comparison of two supplemented aerobic and anaerobic media in automated blood culture systems to detect bloodstream infections. J Clin Microbiol. 1998; 36(3):657-661.

Pohlman JK, Kirkley BA, Easley KA, Basille BA, Washington JA. Controlled clinical evaluation of BACTEC Plus Aerobic/F and BacT/ALERT® aerobic FAN® bottles for detection of bloodstream infections. J Clin Microbiol. 1995;33(11):2856-2858.

Jorgensen JH, Mirrett S, McDonald LC, Murray PR, Weinstein MP, Fune J, Trippy CW, Masterson M, Reller LB. Controlled clinical laboratory comparison of BACTEC Plus Aerobic/F Resin medium with BacT/ALERT® Aerobic FAN® medium for detection of bacteremia and fungemia. J Clin Microbiol. 1997;35(1):53-58.

FAN® Plus Media

Altun O, Almuhayawi M, Ullberg M and Ozenci V, Clinical Evaluation of the FilmArray Blood Culture Identification Panel in Identification of Bacteria and Yeasts from Positive Blood Culture Bottles, J. Clin. Microbiol. 2013, 51(12):4130.

Amarsy-Guerle R, Mougari F, Jacquier H, Oliary J, Benmansour H, Riahi J, Bercot B, Raskine L and Cambau E. High medical impact of implementing the new polymeric bead-based BacT/ALERT® FA Plus and FN Plus blood culture bottles in standard care, Eur J Clin Microbiol Infect Dis. 2015 Feb. Doi: 10.1007/s10096-015-2319-8

Kirn TJ, Mirrett S, Reller LB, Weinstein MP, Controlled Clinical Comparison of BacT/ALERT FA Plus and FN Plus Blood Culture Media with BacT/ALERT FA and FN Blood Culture Media, J. Clin. Microbiol. doi:10.1128/JCM.03063-13.

Lee DH, Kim SC, Bae IG, Kim S, Clinical Evaluation of BacT/Alert FA Plus and FN Plus Bottles Compared with Standard Bottles, J. Clin. Microbiol. 2013, 51(12):4150.

Mitteregger D, Barousch W, Nehr M, Kundi M, Zeitlinger M , Makristathis A and Hirschl AM, Neutralization of Antimicrobial Substances in New BacT/Alert FA and FN Plus Blood Culture Bottles, J. Clin. Microbiol. 2013, 51(5):1534.

Doern C, Mirrett S, Halstead D, Abid J, Okada P and Reller B. Controlled Clinical Comparison of New Pediatric Medium with Adsorbent Polymeric Beads (PF Plus) versus Charcoal-Containing PF Medium in the BacT/Alert Blood Culture System. J. Clin. Microbiol. 2014 52(6): 1898-1900.

FAN® Plus Media vs. BACTEC Plus

Fiori B, D'Inzeo T, Di Florio V, De Maio F, De Angelis G, Giaquinto A, Campana L, Tanzarella E, Tumbarello M, Antonelli M, Sanguinetti M, Spanu T. Performance of Two Resin-Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems. J. Clin. Microbiol. 2014 52(10): 3558-3567

Standard Media Recovery

Mirret S, Reller LB, Petti CA, Woods CW, Vazirani B, Sivadas R, and Weinstein MP. Controlled Clinical Comparison of BacT/ALERT® Standard Aerobic Medium with BACTEC Standard Aerobic Medium for Culturing Blood. J. Clin Microbiol. 2003; 41(6):2391-2394.

Bernard D, Verschraegen G, Claeys G, Lauwers S, Rosseel P. Bilophila wadsworthia bacteremia in a patient with gangrenous appendicitis. Clin Infect Dis. June 1994;18:1023-1024.

Body Fluids

Bourbeau P, Riley J, Heiter BJ, Master R, Young C, Pierson C. Use of the BacT/ALERT® blood culture system for culture of sterile body fluids other than blood. J Clin Microbiol. 1998;36(11):3273-3277.

Alfa MJ, Degagne P, Olson N, Harding KM. Improved detection of bacterial growth in continuous ambulatory peritoneal dialysis effluent by use of BacT/ALERT® FAN® bottles. J Clin Microbiol. 1997;35(4):862-866.

Glass vs. Plastic Bottles

Snyder JW, Munier GK, Bostic GD, Bozigar PS, Hanna R. Evaluation of a Plastic Nonvented Aerobic Blood Culture bottle for Use with the BacT/ALERT® Microbial Detection System. J Clin Microbiol 2002; 40(12): 4757-4759.

Petti, CA, Mirrett S, Woods CW, and Reller LB. Controlled Clinical Comparison of Plastic versus Glass Bottles of BacT/ALERT® PF Medium for Culturing Blood from Children. J Clin Microbiol 2005; 43(1): 445–447

Petti CA, Mirrett S, Woods CW, Reller LB, Controlled clinical comparison of plastic and glass bottles of BacT/ALERT® FA medium for culturing organisms from blood of adult patients. J Clin Microbiol 2005; 43(4), 1960-1962

Pediatric

Alpern E, Alessandrini E, Bell L, Shaw K, McGowan L. Occult bacteremia from a pediatric emergency department: current prevalence, time to detection, and outcome. Pediatrics. 2000;106(3):505-511.

McGowan K, Foster J, Coffin S. Outpatient pediatric blood cultures: time to positivity. Pediatrics. 2000;106(2):251-255.

Krishner KK, Gibb P, CorbettS, and Church DEIDRE. Comparison of the BacT/ALERT® PF Pediatric FAN® Blood Culture Bottle with the Standard Pediatric Blood Culture Bottle, the Pedi-BacT J Clin Microbiol. 2001;39(8): 2880-2883.

BacT/ALERT® vs. ESP

Welby-Sellenriek PL, Keller DS, Ferrett RJ, Storch GA. Comparison of the BacT/ALERT® FAN® aerobic and the Difco ESP 80A aerobic bottles for pediatric blood cultures. J Clin Microbiol. 1997;35(5):1166-1171.

Doern G, Barton A, Rao S. Controlled comparative evaluation of BacT/ALERT® FAN® and ESP 80A aerobic media as means for detecting bacteremia and fungemia. J Clin Microbiol. 1998; 35(9):2686-2689.

Mycobacteria, Yeast and Fungi

Alcaide F, Benitez MA, Escriba JM, Martin R. Evaluation of the BACTEC MGIT™ 960 and the MB/BacT systems for recovery of mycobacteria from clinical specimens and for species identification by DNA AccuProbe. J Clin Microbiol. 2000;38(1):398-401.

Crump J, Tanner DC, Mirrett S, McKnight CM, Reller LB, Controlled Comparison of BACTEC 13A, Myco/F Lytic, BacT/ALERT® MB and Isolator 10 Systems for Detection of Mycobacteremia. J Clin Microbiol 2003;41(5):1987-1990.

McDonald, L., Weinstein, M., Fune, J., Mirrett, S., Reimer, L., Reller, B., Controlled Comparison of BacT/ALERT® FAN® Aerobic Medium and Bactec Fungal Blood Culture Medium for Detection of Fungemia, J Clin Microbiol 2001:39(2): 622–624.

Other

Bourbeau P and Foltzer M. Routine Incubation of BacT/ALERT FA and FN Blood Culture Bottles for More than 3 Days May Not Be Necessary. J Clin Microbiol. 2005: 43(5): 2506-2509.

Seegmuller I, Eschenbach U, Kamereck K. Sensitivity of the BacT/ALERT® FA medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature dependence. J Med Microbiol, (2004); 53. 869-874.

 

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