easySTREAM™

Automated PCR setup from easyMAG® eluates

The easySTREAM™ instrument is a compact pipetting instrument for any laboratory requiring fully automated setup of real-time PCR amplification reactions and sample traceability.

The system can prepare a 96-well PCR reaction plate from nucleic acid eluates in either easyMAG™ vessels or storage tubes, or a combination of both, in ~35 minutes.

easySTREAM™ Overview

The easySTREAM™ instrument is a compact pipetting instrument for any laboratory requiring fully automated setup of real-time PCR amplification reactions and sample traceability. The system can prepare a 96-well PCR reaction plate from master mixes and nucleic acid eluates presented in either easyMAG™ vessels or storage tubes, or from both, in about 35 minutes.

easySTREAM™ is designed to prepare multiple quantitative and qualitative real-time PCR assays. Intelligent pipetting from a test request file allows processing of samples requiring varying subsets of master mixes without re-programming the instrument.

The system includes a barcode reader, UV light for decontamination, and provides a positive pressure HEPA-filtered atmosphere.

easySTREAM™ Uses

  • easySTREAM™ is designed to prepare multiple quantitative and qualitative real-time PCR assays.
  • Enables more productivity from your personnel
  • Less demanding workflow for your staff
  • Frees prep time that can get the thermocycler started earlier
  • Provides traceability to support accreditation and peace of mind

 

Supported thermocyclers and associated consumables
Life Technologies (ABI) 7500: MicroAmp® Optical 96-Well reaction Plate
7500: FastMicroAmp® Fast Optical 96-Well Reaction Plate
Bio-Rad Laboratories CFX96 Real-Time: Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates
Roche LightCycler 480 II: LightCycler® 480 Multiwell Plate 96, white
QIAGEN Rotor-Gene Q: Rotor-Disc 72, Strip Tubes and Caps, 0.1 ml, PCR Tubes, 0.2 ml

 

easySTREAM™ Features

  • Combine multiple quantitative and/or qualitative real-time assays per plate
  • Supports frequently-used thermocycler consumables from ABI, Roche, BioRad and Qiagen
  • Software that thinks like you do: combine samples plus master mixes to create reactions
  • Intelligent pipetting from .csv files: daily workflow involves no re-programming
  • Flexibility to run commercial assays and your laboratory-developed tests
  • Magnetic racks hold easyMAG® eluates stable during pipetting
  • UV sterilization: UV light system for desk sterilization under closed lid conditions
  • HEPA-Filter: High Efficiency Particulate Air Filter system provides positive pressure
  • Cooled Reagent and PCR Plate Holders

easySTREAM™ Specifications

  • Compact benchtop automated liquid handler
  • 23.6” W x 20.3” D x 18.5” H
  • Disposable aerosol-barrier tips, 50 μl and 200 μl
  • 5 - 190 μl pipetting range
  • Single channel, air filled
  • Uses magnetic racks that hold easySTREAM eluates stable during pipetting.
  • Up to 6 easyMAG vessels = 48 input eluates/per plate (in standard configuration)
  • 24 passively-cooled positions for master mixes or controls
  • Cooled position for PCR reaction plate
  • Additional 64 tube positions for additional input elutes or destination storage tubes
  • Supports frequently-used thermocycler consumables from ABI, Roche, BioRad and Qiagen

 

Parameters easySTREAM™
Year Introduced to Market 2014 (Europe); 2015 (US)
Technology Type Air based pipetting system + pLLS (pressure Liquid Level Sensing)
Instrument Footprint
(W x D x H)
Width = 600 mm - Depth = 515 mm - Height = 470 mm
Weight 45 Kg
Operating Ambient Humidity Relative humidity : 20 to 80%
Operating Temperature Range 18 to 25°C
Electrical 100-250 VAC, 50/60 Hz, 60 watts
Maximum Altitude Installation 1000 m
Noise Output in Decibels (dB) 42,5 dB(A)
Table Capacity 9x microplates full positions and 3 reagent racks positions
Pipetting head Single channel , exchangeable head
Pipetting tips 96 racked filtered tips : 50µl, 200 µl
Accuracy Single dispense (50µl tips; dry_well transfer):  5µl from 1.5 mL Microtubes (Trueness < 6% ; CV < 6 %)
10μl from 1.5 mL Microtubes (Trueness < 5% ; CV < 3 %)
10 μl from easyMAG vessels (Trueness < 1% ; CV < 3 %)
Single dispense (200µl tips; dry_well transfer):  180 μl from 1.5 mL Microtubes (Trueness < 2% ; CV < 1 %) 
Multi dispenses (200µl tips; dry_well transfer):  15 μl from ARGENE Master mix tubes (Trueness < 1% ; CV < 3 %) (specific pre-characterized Sarstedt tubes)
Master mix on board Up to 3 x 8 cooled reagents/control tube positions. For ARGENE assays or similar, up to 3 quantitative assays, up to 6 qualitative assays
Time to Result (minutes) 35 min (depending of the number of master mixes used)
Walkaway Time in Minutes 30 min
On-board Sample Capacity 96 eluates
Add Samples with no Pause No
Minimum / Maximum Sample Volume From 15 to 1500 µl
Type of Tubes accepted easyMAG vessels, 0,2 to 1.5 mL microtubes
Dead Volume easyMAG vessels: < 10µl
master mix Simport tubes: < 40µl for multidispense 
QS Simport tubes: <15 µl 
Pre-characterized & approved microtubes 1.5 ml: < 15 µl
STAT Handling Method No
Carryover/Contamination Prevention Force Tips change option  
HEPA Filter
UV Lamp
Sample/PCR mixes Clot Detection Not applicable (eluate)
Sample Liquid Level Sensing Yes
On-board Reagent Refrigeration No – Cooling blocks available for certain reagent vessels and PCR microplates
Auto Calibration No
Software Inventory Management No
Automatic Maintenance Procedure No
Daily Maintenance (min) < 10 min
Technical Support via Modem or Intranet Possible (Vilink)
Remote Diagnostics No
On-Line User's Manual Yes (bioMérieux technical library)

 

easySTREAM™ Performance

easySTREAM™ vs. manual pipetting comparison

Quantification Standard EBV (5x104 copies/reaction)

Manual vs. easySTREAM™ pipetting comparison

 

Pioneering diagnostics