BIOBALL®

Delivering Confidence in Quantitative Microbiology

BIOBALL is a small water-soluble ball containing a precise number of microorganisms delivering unprecedented accuracy for quantitative microbiological quality control. BIOBALL is easy to use and requires no preparation or pre-incubation and is an accredited reference material under ISO Guide 34 standards.

BIOBALL is a small water-soluble ball containing a precise number of microorganisms delivering unprecedented accuracy for quantitative microbiological quality control. BIOBALL is easy to use and requires no preparation or pre-incubation and is an accredited reference material under ISO Guide 34 standards.

Applications include:

  • Media quality control
  • Positive controls for quantitative applications
  • Method validation
  • Accreditation compliance
  • Multi-laboratory standardization

BIOBALL is available at three standard levels:

  • BIOBALL® SINGLESHOT – designed for a single inoculation with unprecedented precision
  • BIOBALL® MULTISHOT - 550 – designed for 10 inoculations with low variance
  • BIOBALL® HIGHDOSE - 10K – designed for a single precise inoculation at a higher CFU level
  • BIOBALL® MULTISHOT - 10E8 – designed for rehydration into 1.1mL of BIOBALL Re-Hydration
  • BIOBALL® PLANT ISOLATE is a new service offering.  It allows you to order BIOBALL with any wild-type strain including environmental monitoring isolates, contaminants of products or raw materials.

USES

  • Quantitative Growth Promotion
  • Method Validation
  • Antimicrobial Activity
  • Positive Controls

BIOBALL FEATURES & SPECS

BIOBALL® SINGLESHOT:

  • Each BIOBALL has a batch mean of between 28 and 33 CFU.
  • Standard deviation less than 3 CFU.
  • BIOBALL SINGLESHOT is ideal when only one or a few inoculations are used within the day or if the highest level of precision is required.

BIOBALL® MULTISHOT - 550:

  • Each BIOBALL has a batch mean of between 500and 600 CFU with a standard deviation <10% of the mean
  • When rehydrated with 1.1 ml of rehydration fluid, provides 10 x 100 uL doses, each containing 50 CFU.
  • 8 hours stability when rehydrated with MULTISHOT - 550 Rehydration Fluid and stored between 2oC and 8oC.
  • Ideal when several controls are needed at the same time or within the same day.

BIOBALL® HIGHDOSE - 10K:

  • Each BIOBALL has a batch mean of between 8000 and 12000 CFU with a standard deviation of <15% of mean
  • Designed for single, precise inoculation
  • Ideal positive control where a higher inoculation level is required

BIOBALL® MULTISHOT - 10E8: 

  • Mean of between 0.7 and 1.5 x 10e8 cfu with a standard deviation of ≤ 20% of the mean.  
  • Designed for rehydration into 1.1 mL of BIOBALL Re-Hydration Fluid to provide 10 x 100 µL doses of 10e7.

BIOBALL® PLANT ISOLATE:

  • BIOBALL PLANT ISOLATE is a new service offering, allowing you to order BIOBALL with any wild-type strain from your in-house collection, whether they're environmental monitoring isolates or contaminants of products or raw materials.
  • BIOBALL PLANT ISOLATE is manufactured at BTF (a bioMérieux company) in Sydney, Australia and maintains the high level of quality you are accustomed to with standard BIOBALL products

BIOBALL is an accredited reference material under ISO Guide 34 standard.

Visit www.bioball.com.

BIOBALL USES

For Agri/Food:

  • Media quality control
  • Quantitative inoculant for the positive control
  • Method validation
  • Proficiency testing
  • Measurement of Uncertainty (MOU) determination
  • Determining limits of detection

BIOBALL needs no preparation prior to use and is water soluble. It can be taken directly from the freezer and added to a media plate, enrichment broth or food sample.

Pathogen Testing – BIOBALL® SINGLESHOT:

Food pathogens (e.g. Salmonella, Listeria monocytogenes, E. coli 0157) can be fatal at very low levels and food test methods need to detect pathogens at these very low levels.

BIOBALL SINGLESHOT is a perfect inoculant for the positive control in pathogen testing. BIOBALL can be added directly to enrichment broth or it can be added as a matrix spike to a sample of sterile matrix and enrichment broth (typically 25 g of sterile matrix and 225 mL of enrichment broth).

BIOBALL can also be made with 5 cfu for those wishing to challenge the limits of detection of their pathogen test method.

Non-Pathogen Testing – BIOBALL® HIGHDOSE - 10K:

Non-pathogen food contaminants such as yeast, mold and some bacteria, if present, are likely to be in large numbers.

BIOBALL HIGHDOSE - 10K contains 10,000 cfu per BIOBALL. It is an ideal inoculant for the positive control in non-pathogen food testing.

BIOBALL HIGHDOSE - 10K can be added as a matrix spike directly to a sample of sterile matrix and diluent (typically 10 g of sterile matrix and 90 mL of diluent) or it can be added directly to diluent (typically 100 mL).

For BioPharma:

  • Media growth promotion
  • Method validation
  • Antimicrobial activity

BIOBALL PRODUCT DEMOS

BIOBALL® SINGLESHOT Demo

BIOBALL® MULTISHOT - 550 Demo

BIOBALL® MULTISHOT - 10E8 Demo

 

Pioneering diagnostics