GENE-UP^®

GENE-UP Overview

In today’s ever-evolving food marketplace, nothing is more important than product safety. To protect your customers, your company and your reputation, you need a fast, simple method to detect, analyze, and make confident decisions.

How GENE-UP Works

Step 1: Sample Preparation and Enrichment

• Standardized protocol and workflow
• Single enrichment
• Incubation time between 8-24 hours

Step 2: Simplified Lysis

• Optimized productivity with Magic Cap
• 5-minute mechanical lysis

Step 3: Amplification and Detection

• Same PCR run for all parameters
• Accurate results within one hour
• Higher level of specificity
• Real-time PCR analysis coupled with end-point melt peak analysis

GENE-UP Benefits

FRET technology & Melt analysis

• Increase profitability by reducing product waste
• Decide with confidence by lessening the number of questionable results
• Unrivaled specificity

Rapid results

• Speed up your decision process
• Improve your manufacturing efficiency
• Decrease your storage cost
• Increase sales profit

Ease of use

UNIQUE & PATENTED lysis reagent

5 minute generic lysis step

• Improve Productivity
• Facilitate lab workflow
• Manage pick of activities
• Decrease cross contamination risk
• No need to work in batches (i.e. Gram + & Gram – follow the same lysis step)

Limited training required

Flexibility

Remote routine software

• Speed-up your decision process

Generic Lysis step

• Facilitate lab organization & workflow

Room temperature storage

Open platform

• Answer to your alternative testing needs (including foodborne viruses)

Reinforce quality & secure data

• Make lab data management real thanks to CONNECT-UP™

GENE-UP Features

Simple to use

• DNA extraction in 5 minutes with generic lysis step
• Patented “Magic Cap” eliminates capping and de-capping, improving productivity
• Room temperature storage of reagents
• Standardized workflow and protocols

Superior performance

• Rapid and reliable results
• High specificity thanks to the primers and probes of FRET technology and melt analysis
• Internal controls in every test
• ​AOAC® RI Performance Tested Methods℠ validated for Salmonella, Cronobacter, Listeria spp., Listeria monocytogenes, E. coli O157:H7 and STEC stx & eae (EH1) and Top 6 (EH2) [EHEC Series]
• GENE UP^® Salmonella (Ref. 414150), GENE UP^® Cronobacter (Ref. 421920), GENE UP^® Listeria spp (Ref. 414059) and GENE UP^® Listeria monocytogenes (Ref. 414058) methods are NF VALIDATION validated by AFNOR Certification, according to ISO 16140-2 standard. GENE UP^® E. coli O157 :H7 (Ref. 414152) method has been validated by Microval according to ISO 16140-2 standard. The scope of those methods is available from our reagent package inserts.

Maximum flexibility

• Compatible lysis and PCR for all parameters
• Anywhere, anytime reporting with remote, web-based software
• Opportunity to design and run homebrew PCR tests
• Capability to run CEERAMTOOLS™ foodborne viruses extended offer

GENE-UP Specs

The GENE-UP instrument can be positioned on any stable and non-moving bench. A typical installation requires workspace of: width 40” and a bench depth 24” and a clearance height of 36”.

GENE-UP Assays

GENE-UP offers ready-to-use test kits for your most pressing food safety and quality needs. Current assays include:

• GENE-UP QUANT Salmonella
  Direct quantification of Salmonella spp 
  → Download assay flyer
• GENE-UP LIS2
  Listeria spp.
• GENE-UP LMO2
  Listeria monocytogenes
• GENE-UP ECO2
  E. coli O157:H7
• GENE-UP STEC
  STEC (stx & eae) EH1
• GENE-UP PEC
  Pathogenic E. coli 
  → Download assay flyer

• GENE-UP EH2
  Top 6 EHEC (E. coli O26, O45, O103, O111, and O145)

• GENE-UP CAM
  Campylobacter spp.
  → Download assay flyer
• GENE-UP CRO
  Cronobacter spp.
• GENE-UP AspergillusPRO
  Detect 4 Aspergillus pathogenic species of concern
  → Download assay flyer
• GENE-UP NUTRAPLEX PRO
  USP Multiplex assay for E. coli, S. aureus, Salmonella
  → Download assay flyer
• GENE-UP STEC / Salmonella
  Simultaneous multiplex detection
  → Download assay flyer
• GENE-UP PRO BEVERAGE
  Multiplex detection for high acid beverage spoilage bacteria and yeast
  → Download assay flyer
• DNA Removal
  BACTIVIAB PMAxx
  → Download Technical Paper
• Food Virus Detection Kits

The Salmonella, Listeria spp., Listeria monocytogenes, E. coli O157:H7 and STEC stx & eae (EH1) and Top 6 (EH2) [EHEC SERIES] assays have received AOAC Performance Tested Methods^SM certification from the AOAC Research Institute (AOAC-RI):

• Salmonella Certificate No. AOAC PTM 121802; OMA 2020.02
• E. coli O157:H7 Certificate No. AOAC PTM 121805; OMA 2019.03
• QUANT Salmonella AOAC PTM 061801
• Listeria spp. Certificate No. AOAC PTM 121803; OMA 2019.10
• Listeria monocytogenes Certificate No. AOAC PTM 121804 OMA 2019.11
• EHEC Series - STEC stx & eae (EH1) and Top 6 (EH2) Certificate No. AOAC PTM 121806 OMA 2020.06
• Cronobacter Certificate No. AOAC PTM 081801 OMA 2019.01

The Salmonella, Cronobacter, Listeria spp., Listeria monocytogenes, and E. coli O157:H7 assays have been validated according to ISO 16140-2 standard.

• Salmonella AFNOR Certificate No. BIO-12/38-06/16
• Listeria spp. AFNOR Certificate No. BIO-12/39-09/16
• Listeria monocytogenes AFNOR Certificate No. BIO-12/40-11/16
• E. coli O157:H7 MicroVal Certificate No. 2015LR59
• Cronobacter ANFOR Certificate No. BIO-12/42-03/18

In addition to our test kits, GENE-UP has the flexibility to perform CEERAMTOOLS® kits for virus & parasite detection and simplex tests.

• Norovirus GI
• Norovirus GII
• Hepatitis A
• Hepatitis E
• Mengovirus
• SARS-CoV-2 from Invisible Sentinel, a bioMérieux company
• More kits also available

CEERAMTOOLS® for GENE-UP

In addition to our test kits, GENE-UP has the flexibility to perform CEERAMTOOLS kits for virus and parasite detection and simplex tests.

Food and Environmental Samples

List of Available CEERAMTOOLS Detection Kits

CEERAMTOOLS Norovirus and Hepatitis A Virus RT-PCR real time kits comply with ISO/TS 15216-1 and 2

The University of Barcelona's Agency of Valorisation and Commercialisation of Research Results (AVCRI) granted two exclusive license agreements with CEERAM:

• Method for detecting HAV using real-tim RT-PCR
• Analytical method that includes Megovirus as a control of the process
   

List of Available CEERAMTOOLS Quantification Standards

The kits are ready-to-use, contain all the necessary reagents (internal control and external positive and negative controls included), have a 12 month shelf life and barcodes for traceability.

The samples for foodborne virus testing usually split between the following categories:

• Fruits: berries, cherries, pomegranite seeds, fruit preparations
• Produce: spinach, blanched leaf vegetables, read to eat food vegetables
• Environmental samples: sewage water, process water, irrigation water, drinking water, surfaces...
• Herbs and spices
• Shellfish
• Processed foods
• Beverages
• Meat
• Dairy

GENE-UP Software

The GENE-UP solution is commercialized with two different software options:

• Routine: to use GENE-UP tests for the detection of the pathogens of interest (Salmonella, Cronobacter, E. coli O157:H7, Listeria spp., Listeria monocytogenes STEC stx & eae [EH1] and Top 6 [EH2])
• Open: to run the CEERAMTOOLS® virus kits, to create your own tests and determine your own interpretation.

GENE-UP routine software

The GENE-UP routine software is used to:

• set up the plate map
• check the results

GENE-UP routine software home page

The GENE-UP routine software has been designed to be accessible from any PC in the lab AND in the office!

• You do not need to be at the bench to setup your plate map and to check your results
• It can speed up your decision process

To give maximum flexibility, the GENE-UP routine software can be installed on your own PC! The software can be connected to your LIMS.

GENE-UP routine software plate map set up page

GENE-UP routine software results page

PRN 059720 Rev 01.A