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Automated PCR setup from easyMAG® eluates
The ESTREAM instrument is a compact pipetting instrument for any laboratory requiring fully automated setup of real-time PCR amplification reactions and sample traceability.
The system can prepare a 96-well PCR reaction plate from nucleic acid eluates in either easyMAG vessels or storage tubes, or a combination of both, in ~35 minutes.
ESTREAM is designed to prepare multiple quantitative and qualitative real-time PCR assays. Intelligent pipetting from a test request file allows processing of samples requiring varying subsets of master mixes without re-programming the instrument.
The system includes a barcode reader, UV light for decontamination, and provides a positive pressure HEPA-filtered atmosphere.
Supported thermocyclers and associated consumables | |
Life Technologies (ABI) | 7500: MicroAmp® Optical 96-Well reaction Plate 7500: FastMicroAmp® Fast Optical 96-Well Reaction Plate |
Bio-Rad Laboratories | CFX96 Real-Time: Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates |
Roche | LightCycler 480 II: LightCycler® 480 Multiwell Plate 96, white |
QIAGEN | Rotor-Gene Q: Rotor-Disc 72, Strip Tubes and Caps, 0.1 ml, PCR Tubes, 0.2 ml |
Parameters | ESTREAM | |
Year Introduced to Market | 2014 (Europe); 2015 (US) | |
Technology Type | Air based pipetting system + pLLS (pressure Liquid Level Sensing) | |
Instrument Footprint (W x D x H) |
Width = 600 mm - Depth = 515 mm - Height = 470 mm | |
Weight | 45 Kg | |
Operating Ambient Humidity | Relative humidity : 20 to 80% | |
Operating Temperature Range | 18 to 25°C | |
Electrical | 100-250 VAC, 50/60 Hz, 60 watts | |
Maximum Altitude Installation | 1000 m | |
Noise Output in Decibels (dB) | 42,5 dB(A) | |
Table Capacity | 9x microplates full positions and 3 reagent racks positions | |
Pipetting head | Single channel , exchangeable head | |
Pipetting tips | 96 racked filtered tips : 50µl, 200 µl | |
Accuracy | Single dispense (50µl tips; dry_well transfer): | 5µl from 1.5 mL Microtubes (Trueness < 6% ; CV < 6 %) |
10μl from 1.5 mL Microtubes (Trueness < 5% ; CV < 3 %) | ||
10 μl from easyMAG vessels (Trueness < 1% ; CV < 3 %) | ||
Single dispense (200µl tips; dry_well transfer): | 180 μl from 1.5 mL Microtubes (Trueness < 2% ; CV < 1 %) | |
Multi dispenses (200µl tips; dry_well transfer): | 15 μl from ARGENE Master mix tubes (Trueness < 1% ; CV < 3 %) (specific pre-characterized Sarstedt tubes) | |
Master mix on board | Up to 3 x 8 cooled reagents/control tube positions. For ARGENE assays or similar, up to 3 quantitative assays, up to 6 qualitative assays | |
Time to Result (minutes) | 35 min (depending of the number of master mixes used) | |
Walkaway Time in Minutes | 30 min | |
On-board Sample Capacity | 96 eluates | |
Add Samples with no Pause | No | |
Minimum / Maximum Sample Volume | From 15 to 1500 µl | |
Type of Tubes accepted | easyMAG vessels, 0,2 to 1.5 mL microtubes | |
Dead Volume | easyMAG vessels: < 10µl | |
master mix Simport tubes: < 40µl for multidispense | ||
QS Simport tubes: <15 µl | ||
Pre-characterized & approved microtubes 1.5 ml: < 15 µl | ||
STAT Handling Method | No | |
Carryover/Contamination Prevention | Force Tips change option | |
HEPA Filter | ||
UV Lamp | ||
Sample/PCR mixes Clot Detection | Not applicable (eluate) | |
Sample Liquid Level Sensing | Yes | |
On-board Reagent Refrigeration | No – Cooling blocks available for certain reagent vessels and PCR microplates | |
Auto Calibration | No | |
Software Inventory Management | No | |
Automatic Maintenance Procedure | No | |
Daily Maintenance (min) | < 10 min | |
Technical Support via Modem or Intranet | Possible (Vilink) | |
Remote Diagnostics | No | |
On-Line User's Manual | Yes (bioMérieux technical library) |
Quantification Standard EBV (5x104 copies/reaction)
Manual vs. ESTREAM pipetting comparison